Bowserina first appears in Super Mario Galaxy 4 The Ultimate Adventure at the beginning when is with the Koopalings when they invade Peach's Castle. She uses her cane to whack Mario, Luigi, Wario, and Waluigi out into space. She later is a boss in Bowser's airship part 1 where she attack with fire and with her cane. She also appears in the super Mario bros super show in the episode: Do you princess toadstool take this koopa?.
- "Ha Ha Ha! Take that you stupid plumbers!"Super Mario Galaxy 4 The Ultimate Adventure when the koopalings attack Princess Peach's castle
- "Oh is that you again Mario. You managed to defeat my step grandchildren but you will NEVER DEFEAT ME!!!!!!!! LET'S BATTLE!!! I WILL USE MY CANE TO WHACK YOU!!! DON'T WORRY!!!! THIS WILL ONLY HURT YOU A LOT!!!!!!!!!!!!! HAHAHAHAHAHAHAHAHAHAHAHAHA!!"Super Mario Galaxy 4 the Ultimate Adventure before battling Mario.
- "I am no old koopa lady you little plumber boys should mess around with, because I can get very dangerous when I'm ferociously angry. So prepare to meet your doom." Super Mario Galaxy 4 the Ultimate Adventure after her 1st defeat before the 2nd battle.